Anti-Human IRF1 Polyclonal Antibody (HY164014)

Name: Anti-Human IRF1 Polyclonal Antibody
Brand: abinScience
SKU: HY164014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY164014

Description

Anti-Human IRF1 Polyclonal Antibody (HY164014) is a rabbit polyclonal antibody detecting IRF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human IRF1 (Trp11-Ser128).

Target

IRF-1, Interferon regulatory factor 1, Irf1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Observed Molecular Weight

52 kDa

Purification

Purified by antigen affinity column.

Accession

P15314

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interferon regulatory factor 1 (IRF1) is a ~36 kDa protein. Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Has an essentail role in IFNG-dependent immunity to mycobacteria. Competes with the transcriptional repressor ZBED2 for binding to a common consensus sequence in gene promoters.

1. Oshima, S. et al. (2004) Molecular and cellular biology 24, 6298-310. PMID: 15226432

2. Dornan, D. et al. (2004) Molecular and cellular biology 24, 10083-98. PMID: 15509808

3. Su, ZZ. et al. (2007) Journal of cellular physiology 213, 502-10. PMID: 17516545

4. Park, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 17028-33. PMID: 17942705

5. Bowie, ML. et al. (2008) Advances in experimental medicine and biology 617, 367-74. PMID: 18497060

6. Huang, Y. et al. (2009) PloS one 4, e5397. PMID: 19404407

7. Gao, J. et al. (2010) Cell death and differentiation 17, 699-709. PMID: 19851330

8. Qi, H. et al. (2012) The Journal of biological chemistry 287, 12622-33. PMID: 22367195

9. Somerville, TDD. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 11471-11482. PMID: 32385160

10. Tanaka, N. et al. (1993) Molecular and cellular biology 13, 4531-8. PMID: 7687740

Note

For research use only.

Images

References

Datasheet

Anti-Human IRF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IRF1 Polyclonal Antibody (HY164014)

Overview

Images

References

Datasheet

SDS