Mouse IRF1 Recombinant Protein (N-His) (MY164012)

Name: Mouse IRF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MY164012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MY164012
Description	Recombinant Mouse IRF1 Protein, N-His (MY164012) expressed in E. coli, spanning Trp11-Ser128. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P15314
Protein length	Trp11-Ser128
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.19 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Interferon regulatory factor 1, IRF-1, Irf1
Background	Interferon regulatory factor 1 (IRF1) is a ~37 kDa protein. Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Has an essentail role in IFNG-dependent immunity to mycobacteria. Binds to a consensus sequence in gene promoters. 1. Taniguchi, T. et al. (2001) Annual review of immunology 19, 623-55. PMID: 11244049 2. Kröger, A. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 5-14. PMID: 11846971 3. Romeo, G. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 39-47. PMID: 11846974 4. Honda, K. et al. (2006) Nature reviews. Immunology 6, 644-58. PMID: 16932750 5. Savitsky, D. et al. (2010) Cancer immunology, immunotherapy : CII 59, 489-510. PMID: 20049431 6. Man, SM. et al. (2015) Nature immunology 16, 467-75. PMID: 25774715
Note	For research use only.

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SDS-PAGE for Recombinant Mouse IRF1 Protein, N-His

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Datasheet

Mouse IRF1 Recombinant Protein (N-His).pdf

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Mouse IRF1 Recombinant Protein (N-His) (MY164012)

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