Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043)

Name: Anti-Human B-Raf Recombinant Antibody (SRR0002)
Brand: abinScience
SKU: HB617043
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HB617043
Description	Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043) is a rabbit monoclonal antibody detecting B-Raf in IF, WB. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	IF, WB
Host species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone ID	SRR0002
Tested applications	IF, WB
Target	BRAF, BRAF1, EC:2.7.11.1, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P15056
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. 1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910 2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126 3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789 4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179
Note	For research use only.

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Western blot

Western blot analysis was performed using anti-B-Raf monoclonal antibody at 1 ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HEK293T cell lysate
Immunofluorescence

HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting B-Raf (HB617043) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of B-Raf was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet

Anti-Human B-Raf Recombinant Antibody (SRR0002).pdf

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Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043)

Overview

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References

Datasheet

SDS