Anti-KRAS Polyclonal Antibody (HF904014) is a rabbit polyclonal antibody detecting KRAS in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Artemia salina, and Carassius auratus.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
GTPase KRas (K-Ras 2) is a ~21 kDa protein. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.
1. Gremer, L. et al. (2011) Human mutation 32, 33-43. PMID: 20949621
2. Bernal Astrain, G. et al. (2025) Nature communications 16, 647. PMID: 39809765
3. Yang, MH. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 10843-8. PMID: 22711838
4. Zimmermann, G. et al. (2013) Nature 497, 638-42. PMID: 23698361
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