Anti-Human Integrin beta 1/CD29 Polyclonal Antibody (HY248014)

Name: Anti-Human Integrin beta 1/CD29 Polyclonal Antibody
Brand: abinScience
SKU: HY248014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY248014

Description

Anti-CD29/ITGB1 Polyclonal Antibody (HY248014) is a rabbit polyclonal antibody detecting ITGB1 in ELISA, IHC, WB. Suitable for Human, Mouse, Cat, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CD29/ITGB1 (Gln21-Asp728).

Tested applications

Target

MSK12, VLA-4 subunit beta, CD29, Integrin beta-1, ITGB1, FNRB, MDF2, GPIIA, Glycoprotein IIa, Fibronectin receptor subunit beta

Purification

Purified by antigen affinity column.

Accession

P05556

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Integrin beta-1 (CD29/ITGB1) is a ~88 kDa protein. Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen.

1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415

2. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099

3. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536

4. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877

5. Bax, DV. et al. (2003) The Journal of biological chemistry 278, 34605-16. PMID: 12807887

6. Jovanovic, J. et al. (2007) The Journal of biological chemistry 282, 6743-51. PMID: 17158881

7. Schumacher, S. et al. (2021) Science advances 7. PMID: 33962943

8. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 29030430

9. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973

10. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ITGB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HUVEC cell lysate
Lane 3: Mouse spleen lysate
Lane 4: Rat spleen lysate