Anti-CD10/MME Polyclonal Antibody (HY401014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HY401014

Description

Anti-CD10/MME Polyclonal Antibody (HY401014) is a rabbit polyclonal antibody detecting CD10 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pongo abelii, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Oryctolagus cuniculus, Pongo abelii, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD10/MME (Met1-Cys80).

Target

Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN

Purification

Purified by antigen affinity column.

Accession

P08473

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)).

1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675

2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535

3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683

4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535

5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991

6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD10/MME polyclonal antibody at 1ug/mL on recombinant CD10/MME (Catalog No. HY401012).

References

Datasheet

Anti-CD10/MME Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD10/MME Polyclonal Antibody (HY401014)

Overview

Images

References

Datasheet

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