Anti-Human CCL2/MCP-1 Polyclonal Antibody (HY280014)

Name: Anti-Human CCL2/MCP-1 Polyclonal Antibody
Brand: abinScience
SKU: HY280014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HY280014

Description

Anti-CCL2/MCP-1 Polyclonal Antibody (HY280014) is a rabbit polyclonal antibody detecting MCP-1 in ELISA, IHC, WB. Suitable for Human, Macaca fascicularis, Macaca mulatta, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CCL2/MCP-1 (Gln24-Thr99).

Target

HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2

Purification

Purified by antigen affinity column.

Accession

P13500

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

C-C motif chemokine 2 (CCL2/MCP-1) is a ~11 kDa protein. Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

1. Hemmerich, S. et al. (1999) Biochemistry 38, 13013-25. PMID: 10529171

2. Jarnagin, K. et al. (1999) Biochemistry 38, 16167-77. PMID: 10587439

3. Paavola, CD. et al. (1998) The Journal of biological chemistry 273, 33157-65. PMID: 9837883

4. Zhang, YJ. et al. (1994) The Journal of biological chemistry 269, 15918-24. PMID: 8195247

5. Weber, M. et al. (1996) The Journal of experimental medicine 183, 681-5. PMID: 8627182

6. Chakravarty, L. et al. (1998) The Journal of biological chemistry 273, 29641-7. PMID: 9792674

7. Li, YS. et al. (1993) Molecular and cellular biochemistry 126, 61-8. PMID: 8107690

Note

For research use only.

Images

References

Datasheet

Anti-Human CCL2/MCP-1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human CCL2/MCP-1 Polyclonal Antibody (HY280014)

Overview

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References

Datasheet

SDS

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