Anti-BRAF Polyclonal Antibody (HB617014)

Name: Anti-BRAF Polyclonal Antibody
Brand: abinScience
SKU: HB617014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB617014

Description

Anti-BRAF Polyclonal Antibody (HB617014) is a rabbit polyclonal antibody detecting BRAF in ELISA, IHC, WB. Suitable for Coturnix japonica, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Avian retrovirus IC10, Avian rous-associated virus type 1, Coturnix japonica, Chicken, Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BRAF (Asn500-His766).

Target

BRAF1, RAFB1, Proto-oncogene B-Raf, BRAF, v-Raf murine sarcoma viral oncogene homolog B1, p94, Serine/threonine-protein kinase B-raf

Purification

Purified by antigen affinity column.

Accession

P15056

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons.

1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910

2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126

3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789

4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179

Note

For research use only.

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