Anti-MCM5 Polyclonal Antibody (HW592014)

Name: Anti-MCM5 Polyclonal Antibody
Brand: abinScience
SKU: HW592014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW592014

Description

Anti-MCM5 Polyclonal Antibody (HW592014) is a rabbit polyclonal antibody detecting MCM5 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Xenopus tropicalis, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MCM5 (Val331-Ile537).

Target

CDC46, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5

Purification

Purified by antigen affinity column.

Accession

P33992

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA replication licensing factor MCM5 is a ~82 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Li, Z. et al. (2025) The EMBO journal 44, 6019-6050. PMID: 40940420

2. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

Note

For research use only.

Images

References

Datasheet

Anti-MCM5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-MCM5 Polyclonal Antibody (HW592014)

Overview

Images

References

Datasheet

SDS

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