Anti-NOS2 Polyclonal Antibody (HW388014)

Name: Anti-NOS2 Polyclonal Antibody
Brand: abinScience
SKU: HW388014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HW388014

Description

Anti-NOS2 Polyclonal Antibody (HW388014) is a rabbit polyclonal antibody detecting NOS2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NOS2 (Met533-Lys696).

Target

Nitric oxide synthase, inducible, NOS2A, Inducible NO synthase, HEP-NOS, Hepatocyte NOS, Peptidyl-cysteine S-nitrosylase NOS2, iNOS, NOS2, Inducible NOS, NOS type II

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P35228

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nitric oxide synthase, inducible (NOS2/iNOS) is a ~131 kDa protein. Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.

1. Charles, IG. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11419-23. PMID: 7504305

2. Hokari, A. et al. (1994) Journal of biochemistry 116, 575-81. PMID: 7531687

3. Guo, FH. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7809-13. PMID: 7544004

4. Geller, DA. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 3491-5. PMID: 7682706

5. Jia, J. et al. (2014) Cell 159, 623-34. PMID: 25417112

6. Vuolteenaho, K. et al. (2009) Mediators of inflammation 2009, 345838. PMID: 19688109

Note

For research use only.

Images

References

Datasheet

Anti-NOS2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NOS2 Polyclonal Antibody (HW388014)

Overview

Images

References

Datasheet

SDS

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