Anti-F2RL1 Polyclonal Antibody (HX041014)

Name: Anti-F2RL1 Polyclonal Antibody
Brand: abinScience
SKU: HX041014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HX041014

Description

Anti-F2RL1 Polyclonal Antibody (HX041014) is a rabbit polyclonal antibody detecting F2RL1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human F2RL1 (Leu15-Val87).

Target

GPR11, Thrombin receptor-like 1, G-protein coupled receptor 11, F2RL1, PAR-2, Coagulation factor II receptor-like 1, Proteinase-activated receptor 2, PAR2

Purification

Purified by antigen affinity column.

Accession

P55085

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Proteinase-activated receptor 2 (F2RL1/PAR-2) is a ~44 kDa protein. Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2R/PAR1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation.

1. Cheng, RKY. et al. (2017) Nature 545, 112-115. PMID: 28445455

2. Kuckleburg, CJ. et al. (2013) Arteriosclerosis, thrombosis, and vascular biology 33, 275-84. PMID: 23202369

3. Cocks, TM. et al. (1999) Nature 398, 156-60. PMID: 10086357

4. Botham, A. et al. (2011) The Biochemical journal 438, 359-67. PMID: 21627585

5. Manithody, C. et al. (2012) Biochemistry 51, 2551-7. PMID: 22409427

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-F2RL1 polyclonal antibody at 1ug/mL on recombinant F2RL1 (Catalog No. HX041012).

References

Datasheet

Anti-F2RL1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-F2RL1 Polyclonal Antibody (HX041014)

Overview

Images

References

Datasheet

SDS

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