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Anti-SEC11A Polyclonal Antibody (HX979014)

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Overview
Catalog No.HX979014
Description
Anti-SEC11A Polyclonal Antibody (HX979014) is a rabbit polyclonal antibody detecting SEC11A in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human SEC11A (Met1-Glu179).
Target Microsomal signal peptidase 18 kDa subunit, Endopeptidase SP18, SPCS4A, Signal peptidase complex catalytic subunit SEC11A, SEC11A, SPase 18 kDa subunit, SEC11-like protein 1, SEC11 homolog A, SPC18, SEC11L1
Purification Purified by antigen affinity column.
Accession P67812
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Signal peptidase complex catalytic subunit SEC11A is a ~20 kDa protein. Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.

1. Liaci, AM. et al. (2021) Molecular cell 81, 3934-3948.e11. PMID: 34388369
Note For research use only.
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Formula
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Formula
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Stock Solution
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-SEC11A Polyclonal Antibody

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