Anti-Human NOTCH2/3 Antibody (SAA0143) (HC513107)

Flow-cytometry using anti-human NOTCH2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human NOTCH3 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH3 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human NOTCH2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human NOTCH2 & NOTCH3 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 & NOTCH3 antibody monoclonal antibody (Catalog # HC513107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.