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Anti-Human CD13/ANPEP Antibody (CD13-1) (HB650107)

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Anti-Human CD13/ANPEP Antibody (CD13-1)

Anti-Human CD13/ANPEP Antibody (CD13-1)

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Datasheet

Overview

Catalog No.	HB650107
Description	Anti-Human CD13/ANPEP Antibody (CD13-1) [CD13-1] (HB650107) is a mouse monoclonal antibody detecting ANPEP in ELISA, FCM, SPR, WB. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, SPR, WB
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	CD13-1
Clonality	Monoclonal
Target	Aminopeptidase M, APN, AP-M, gp150, hAPN, AP-N, PEPN, Microsomal aminopeptidase, CD13, Aminopeptidase N, Alanyl aminopeptidase, Myeloid plasma membrane glycoprotein CD13, ANPEP
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P15144
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Aminopeptidase N (CD13/ANPEP) is a ~109 kDa protein. Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.
Note	For research use only.

Images

Flow CytoMetry

Flow-cytometry using anti-human CD13 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD13 monoclonal antibody (Catalog: HB650107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD13/ANPEP Antibody (CD13-1).pdf

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Anti-Human CD13/ANPEP Antibody (CD13-1)