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Anti-Human CD2 Antibody (G11) (HY113107)

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  • Datasheet

Overview
Catalog No.HY113107
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDG11
Clonality Monoclonal
Target Erythrocyte receptor, SRBC, T-cell surface antigen CD2, Rosette receptor, CD2, LFA-3 receptor, T-cell surface antigen T11/Leu-5, LFA-2
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P06729
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Bioactivity

    Detects CD2 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human CD2 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD2 antibody monoclonal antibody (Catalog # HY113107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-CD2 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human CD2 (Catalog No: HY113011)
    Lane 2: negative control

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