Anti-Human CD318/CDCP1 Antibody (CUB4) (HV451107)

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Overview

Catalog No.	HV451107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	CUB4
Clonality	Monoclonal
Target	CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9H5V8
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

Detects Human CD318/CDCP1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD318 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 antibody monoclonal antibody (Catalog # HV451107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD318 antibody.SW480 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 antibody monoclonal antibody (Catalog # HV451107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet