Anti-Mouse CD5 Recombinant Antibody (YTS 121.5.2) (MY046107)

Name: Anti-Mouse CD5 Recombinant Antibody (YTS 121.5.2)
Brand: abinScience
SKU: MY046107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	MY046107
Description	Anti-Mouse CD5 Recombinant Antibody (YTS 121.5.2) (MY046107) is a rat monoclonal antibody detecting CD5 in FCM. Suitable for Mouse. Highlights ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivity	Mouse
Applications	FCM
Host species	Rat
Clonality	Monoclonal
Isotype	IgG2b
Clone ID	YTS 121.5.2
Target	CD5, Cd5, Ly-1, Lymphocyte antigen 1, Lyt-1, T-cell surface glycoprotein CD5
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13379
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	T-cell surface glycoprotein CD5 (Lymphocyte antigen T1/Leu-1,CD5,T-cell surface glycoprotein CD5,LEU1) is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. 1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049 2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158 3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399 4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Mouse CD5 Recombinant Antibody (YTS 121.5.2).pdf

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Anti-Mouse CD5 Recombinant Antibody (YTS 121.5.2) (MY046107)

Overview

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References

Datasheet

SDS