Anti-Human ROBO1 Recombinant Antibody (B5209B), PerCP (HP485147)

Name: Anti-Human ROBO1 Recombinant Antibody (B5209B), PerCP
Brand: abinScience
SKU: HP485147
Price: 290 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50T
100T

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HP485147
Description	Anti-Human ROBO1 Recombinant Antibody (B5209B), PerCP (HP485147) is a human monoclonal antibody detecting ROBO1 in FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●PerCP Conjugated — Ready for direct detection without secondary antibody.
Species reactivity	Human
Applications	FCM
Host species	Human
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone ID	B5209B
Conjugation	PerCP
Target	ROBO1, Roundabout homolog 1, H-Robo-1
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9Y6N7
Form	Liquid
Storage buffer	0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Roundabout homolog 1 (ROBO1) is a ~180 kDa protein. Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. 1. Brose, K. et al. (1999) Cell 96, 795-806. PMID: 10102268 2. Leyva-Díaz, E. et al. (2014) Current biology : CB 24, 494-508. PMID: 24560577 3. Kong, R. et al. (2015) The Journal of clinical investigation 125, 4407-20. PMID: 26529257
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

References

Datasheet

Anti-Human ROBO1 Recombinant Antibody (B5209B), PerCP.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ROBO1 Recombinant Antibody (B5209B), PerCP (HP485147)

Overview

Images

References

Datasheet

SDS