Anti-Human CD66b/CEACAM8 Antibody (BW250/183) (HB202107)

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Overview

Catalog No.	HB202107
Description	Anti-Human CD66b/CEACAM8 Antibody (BW250/183) [BW250/183] (HB202107) is a mouse monoclonal antibody detecting CEACAM8 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	BW250/183
Clonality	Monoclonal
Target	CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CGM6, CD66b, Non-specific cross-reacting antigen NCA-95, CEACAM8
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P31997
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Cell adhesion molecule CEACAM8 (CD66b) is a ~38 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. 1. Kuroki, M. et al. (2001) Journal of leukocyte biology 70, 543-50. PMID: 11590190 2. Oikawa, S. et al. (1991) The Journal of biological chemistry 266, 7995-8001. PMID: 2022629 3. Yamanaka, T. et al. (1996) Protein expression and purification 7, 438-46. PMID: 8776764
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD66b/CEACAM8 Antibody (BW250/183).
Flow CytoMetry

Flow-cytometry using anti-human CD66b antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD66b monoclonal antibody (Catalog: HB202107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.