Anti-Human LPL/LIPD Antibody (5D2) (HY363107)

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Overview

Catalog No.	HY363107
Description	Anti-Human LPL/LIPD Antibody (5D2) [5D2] (HY363107) is a mouse monoclonal antibody detecting LIPD in ELISA, FCM, IF, IHC(P), IP, WB. Suitable for Human, Mouse, Rat, and Bovine. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications. ●Multi-Species — Cross-reactive for translational research.
Species reactivity	Human, Mouse, Rat, Bovine
Applications	ELISA, FCM, IF, IHC(P), IP, WB
Host species	Mouse
Isotype	IgG2a
Clone ID	5D2
Clonality	Monoclonal
Target	Phospholipase A1, LIPD, Lipoprotein lipase, LPL
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P06858
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Lipoprotein lipase (LPL/LIPD) is a ~53 kDa protein. Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. 1. Lutz, EP. et al. (2001) The Journal of clinical investigation 107, 1183-92. PMID: 11342582 2. Pingitore, P. et al. (2016) Journal of clinical lipidology 10, 816-823. PMID: 27578112 3. Weinstock, PH. et al. (1995) The Journal of clinical investigation 96, 2555-68. PMID: 8675619 4. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167 5. Dugi, KA. et al. (1995) The Journal of biological chemistry 270, 25396-401. PMID: 7592706 6. Goulbourne, CN. et al. (2014) Cell metabolism 19, 849-60. PMID: 24726386 7. Allan, CM. et al. (2017) Journal of lipid research 58, 216-225. PMID: 27811232
Note	For research use only.

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SDS-PAGE for Anti-Human LPL/LIPD Antibody (5D2)

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Datasheet

Anti-Human LPL/LIPD Antibody (5D2).pdf

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Anti-Human LPL/LIPD Antibody (5D2) (HY363107)

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