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Anti-Human CD54/ICAM1 Antibody (SAA0025), APC (HY321137)

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Anti-Human CD54/ICAM1 Antibody (SAA0025), APC

Anti-Human CD54/ICAM1 Antibody (SAA0025), APC

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Datasheet

Overview

Catalog No.	HY321137
Description	Anti-Human CD54/ICAM1 Antibody (SAA0025), APC [SAA0025] (HY321137) is a human monoclonal antibody detecting ICAM1 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, lambda
Clone ID	SAA0025
Conjugation	APC
Clonality	Monoclonal
Target	ICAM1, Major group rhinovirus receptor, CD54, ICAM-1, Intercellular adhesion molecule 1
Endotoxin level	Please contact with the lab for this information.
Accession	P05362
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Intercellular adhesion molecule 1 (CD54/ICAM1) is a ~57 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM:ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1. 1. Dustin, ML. et al. (1986) Journal of immunology (Baltimore, Md. : 1950) 137, 245-54. PMID: 3086451 2. Simmons, D. et al. (1988) Nature 331, 624-7. PMID: 3340213 3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629 4. Diamond, MS. et al. (1990) The Journal of cell biology 111, 3129-39. PMID: 1980124 5. van Buul, JD. et al. (2007) The Journal of cell biology 178, 1279-93. PMID: 17875742 6. Hayashi, T. et al. (2001) Digestion 63 Suppl 1, 87-92. PMID: 11173916
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using APC anti-human CD54 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD54 monoclonal antibody (Catalog: HY321137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD54/ICAM1 Antibody (SAA0025), APC.pdf

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Anti-Human CD54/ICAM1 Antibody (SAA0025), APC