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Anti-Human CD243/P-Glycoprotein Antibody (C219), APC (HY466137)

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Overview

Catalog No.	HY466137
Description	Anti-Human CD243/P-Glycoprotein Antibody (C219), APC [C219] (HY466137) is a mouse monoclonal antibody detecting P-glycoprotein 1 in FCM, WB. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	C219
Conjugation	APC
Clonality	Monoclonal
Target	Phospholipid transporter ABCB1, MDR1, CD243, ATP-binding cassette sub-family B member 1, ABCB1, Multidrug resistance protein 1, P-glycoprotein 1, ATP-dependent translocase ABCB1, PGY1, P-Glycoprotein, P Glycoprotein
Endotoxin level	Please contact with the lab for this information.
Accession	P08183
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	ATP-dependent translocase ABCB1 (CD243) is a ~141 kDa protein. Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. 1. Choi, KH. et al. (1988) Cell 53, 519-29. PMID: 2897240 2. Masud, SN. et al. (2022) Nature chemical biology 18, 1370-1379. PMID: 35970996 3. van Helvoort, A. et al. (1996) Cell 87, 507-17. PMID: 8898203 4. Chen, G. et al. (1997) The Journal of biological chemistry 272, 5974-82. PMID: 9038218 5. Shafik, SH. et al. (2022) PLoS biology 20, e3001616. PMID: 35507548
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human CD243/P-Glycoprotein Antibody (C219), APC.pdf

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Anti-Human CD243/P-Glycoprotein Antibody (C219), APC