HEK293T Cell Line (HG001818)

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Overview

Catalog No.	HG001818
Description	HEK293T Cell Line (HG001818) is a parental HEK293T cell line. Widely used as a host cell for transient transfection, stable cell line generation, and recombinant protein production. Highlights ●Parental Cell Line — Unmodified HEK293T cells suitable as host cells or negative controls. ●High Transfection Efficiency — Contains the SV40 large T antigen for enhanced episomal replication of plasmids carrying the SV40 origin. ●Versatile Applications — Ideal for transient transfection, lentiviral packaging, stable cell line development, and recombinant protein expression.
Applications	FCM
Species	Human
Host species	HEK293T
Properties	Growth Properties: Adherent; Morphology: Epithelial; Culture Medium: DMEM + 10% FBS + 1% Penicillin-Streptomycin; Culture Conditions: 37°C, 5% CO₂
Shipping	Dry ice
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Alternate Names	293T, HEK-293T, HEK 293T
Background	HEK293T is a highly transfectable derivative of the human embryonic kidney 293 (HEK293) cell line that stably expresses the SV40 large T antigen. The presence of the T antigen allows episomal replication of transfected plasmids containing the SV40 origin of replication, leading to amplification of the plasmid and extended temporal expression of the desired gene products. HEK293T cells are widely used for transient protein expression, lentiviral and retroviral packaging, and as host cells for generating stable cell lines overexpressing specific target genes. They grow as an adherent monolayer and are readily maintained in standard culture conditions.
Note	For research use only