Nipah virus Nucleoprotein Antibody Pair Set (VK432908)

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Datasheet
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Protocol

Overview

Catalog No.	VK432908
Description	Nipah virus Nucleoprotein Antibody Pair Set (VK432908) is a capture: mouse, detection: mouse antibody detecting Nucleoprotein in ELISA. Suitable for Nipah virus (NiV). Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Sensitivity	9.12 ng/mL
Range	31.25 - 2,000 ng/mL
Species reactivity	Nipah virus (NiV)
Host species	Capture: Mouse, Detection: Mouse
Immunogen	Nucleoprotein
Conjugation	Detection: Biotin
Tested applications	S-ELISA
Target	Nucleoprotein
Specificity	Nucleoprotein
Applications	ELISA
Form	Liquid
Storage buffer	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Assay type	Sandwich ELISA
Specifications	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Background	Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.
Note	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Images

Standard Curve

Standard Curve of Nipah virus Nucleoprotein Antibody Pair Set

References

Datasheet

Nipah virus Nucleoprotein Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits