Human GDF15 Antibody Pair Set (HV986908)

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Overview

Catalog No.	HV986908
Description	Human GDF15 Antibody Pair Set (HV986908) is a capture: mouse, detection: mouse antibody detecting GDF-15 in ELISA. Suitable for Human. Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Species reactivity	Human
Host species	Capture: Mouse, Detection: Mouse
Immunogen	GDF-15
Conjugation	Detection: Biotin
Tested applications	S-ELISA
Target	GDF-15
Specificity	GDF-15
Applications	ELISA
Form	Liquid
Storage buffer	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Assay type	Sandwich ELISA
Specifications	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Background	Growth/differentiation factor 15 (GDF15/MIC1) is a ~34 kDa protein. Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea, vomiting, and/or loss of appetite. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues. 1. Tsai, VW. et al. (2013) PloS one 8, e55174. PMID: 23468844 2. Tsai, VW. et al. (2014) PloS one 9, e100370. PMID: 24971956 3. Mullican, SE. et al. (2017) Nature medicine 23, 1150-1157. PMID: 28846097 4. Emmerson, PJ. et al. (2017) Nature medicine 23, 1215-1219. PMID: 28846098 5. Yang, L. et al. (2017) Nature medicine 23, 1158-1166. PMID: 28846099 6. Hsu, JY. et al. (2017) Nature 550, 255-259. PMID: 28953886 7. Xiong, Y. et al. (2017) Science translational medicine 9. PMID: 29046435 8. Patel, S. et al. (2019) Cell metabolism 29, 707-718.e8. PMID: 30639358 9. Coll, AP. et al. (2020) Nature 578, 444-448. PMID: 31875646 10. Klein, AB. et al. (2021) Nature communications 12, 1041. PMID: 33589633
Note	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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References

Datasheet

Human GDF15 Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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