Human IL-8/CXCL8 Antibody Pair Set (HC517908)

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Protocol

Overview

Catalog No.	HC517908
Description	Human IL-8/CXCL8 Antibody Pair Set (HC517908) is a capture: mouse, detection: mouse antibody detecting IL-8/CXCL8 in ELISA. Suitable for Human. Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Sensitivity	3.57 pg/mL
Range	31.25-2,000 pg/mL
Species reactivity	Human
Host species	Capture: Mouse, Detection: Mouse
Immunogen	IL-8/CXCL8
Conjugation	Detection: Biotin
Tested applications	S-ELISA
Target	IL-8/CXCL8
Specificity	IL-8/CXCL8
Applications	ELISA
Form	Liquid
Storage buffer	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Assay type	Sandwich ELISA
Specifications	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Background	Interleukin-8 (IL8/CXCL8) is a ~11 kDa protein. Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. 1. Zinkernagel, AS. et al. (2008) Cell host & microbe 4, 170-8. PMID: 18692776 2. Hammond, ME. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 155, 1428-33. PMID: 7636208 3. Van Damme, J. et al. (1990) European journal of immunology 20, 2113-8. PMID: 2145175 4. Jacobi, HH. et al. (1998) International archives of allergy and immunology 116, 53-9. PMID: 9623510 5. Holmes, WE. et al. (1991) Science (New York, N.Y.) 253, 1278-80. PMID: 1840701 6. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716 7. Takami, M. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 4559-66. PMID: 11971003 8. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Note	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Images

Standard Curve

Standard Curve of Human IL-8/CXCL8 Antibody Pair Set

References

Datasheet

Human IL-8/CXCL8 Antibody Pair Set.pdf

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Protocol

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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