BDBV Nucleoprotein Antibody Pair Set (VK059908)

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Datasheet
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Protocol

Overview

Catalog No.	VK059908
Description	BDBV Nucleoprotein Antibody Pair Set (VK059908) is a capture: rabit; detection: rabit antibody detecting Nucleoprotein in ELISA. Suitable for Bundibugyo virus (BDBV). Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Sensitivity	2.37 ng/mL
Range	7.8 - 500 ng/mL
Species reactivity	Bundibugyo virus (BDBV)
Host species	Capture: Rabbit, Detection: Rabbit
Conjugation	Detection: Biotin
Tested applications	Sandwich ELISA
Target	Nucleoprotein
Specificity	Nucleoprotein
Applications	ELISA
Form	Liquid
Storage buffer	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Detection method	Detection method
Assay type	Sandwich ELISA
Specifications	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Background	Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.
Note	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Images

Standard Curve

Standard Curve of BDBV Nucleoprotein Antibody Pair Set

References

Datasheet

BDBV Nucleoprotein Antibody Pair Set.pdf

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Protocol

Download Protocol PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits