FMDV VP1 Antibody Pair Set (VK500908)

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Datasheet
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Protocol

Overview

Catalog No.	VK500908
Description	FMDV VP1 Antibody Pair Set (VK500908) is a capture: human; detection: rabit antibody detecting VP1 in ELISA. Suitable for Foot-and-mouth disease virus (FMDV). Highlights ●Detection: Biotin Conjugated — Ready for direct detection without secondary antibody.
Sensitivity	1.11 ng/mL
Range	3.1 - 200 ng/mL
Species reactivity	Foot-and-mouth disease virus (FMDV)
Host species	Capture: Human, Detection: Rabbit
Conjugation	Detection: Biotin
Tested applications	Sandwich ELISA
Target	VP1
Specificity	VP1
Applications	ELISA
Form	Liquid
Storage buffer	Capture: 0.01M PBS pH 7.4; Detection: 0.01M PBS pH 7.4, 50% glycerol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Detection method	Detection method
Assay type	Sandwich ELISA
Specifications	Reagents are sufficient for at least 1 x 96 well plates using recommended protocol.
Background	Capsid protein VP1 is a ~81 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s).
Note	For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Images

Standard Curve

Standard Curve of FMDV VP1 Antibody Pair Set

References

Datasheet

FMDV VP1 Antibody Pair Set.pdf

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Protocol

Download Protocol PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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