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Treponema pallidum TPP47 ELISA Kit (JN038018)

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Overview
Catalog No.JN038018
Description
Treponema pallidum TPP47 ELISA Kit (JN038018) — Quantitative sandwich ELISA for Treponema pallidum TPP47. Sample: Plasma, Serum. Range: 1.56 - 100 ng/mL. Sensitivity: 0.65 ng/mL.
Highlights
  • ●Sandwich Format — High specificity, reliable quantification.
  • ●High Sensitivity — Optimized for low-abundance target detection.
  • ●Ready-to-Use — Complete kit with pre-coated plates included.
Sample typeSerum, plasma, and other biological fluid samples
Sensitivity0.71 ng/mL
Range1.56–100 ng/mL
Species reactivity Treponema pallidum
Target Treponema pallidum TPP47
Applications ELISA
Detection method Colorimetric
Assay type Sandwich
Recovery 80-120%
Shipping 2-8 ℃
Stability and Storage The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Specifications

TPP47

Background

Putative DD-carboxypeptidase TP_0574 (TPP47) is a ~47 kDa protein. A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin.

1. Deka, RK. et al. (2002) The Journal of biological chemistry 277, 41857-64. PMID: 12196546
2. Weigel, LM. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 11611-5. PMID: 7972112
3. Radolf, JD. et al. (1989) Infection and immunity 57, 1248-54. PMID: 2647634
Note For research use only.
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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