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Anti-Vaccinia virus(VACV) A33R Recombinant Antibody (A27D7) (VK465013)

Anti-Vaccinia virus(VACV) A33R Recombinant Antibody (A27D7)
Anti-Vaccinia virus(VACV) A33R Recombinant Antibody (A27D7)
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Overview
Catalog No.VK465013
Description
Anti-Vaccinia virus(VACV) A33R Recombinant Antibody (A27D7) (VK465013) is a mouse monoclonal antibody detecting A33R in ELISA, Neutralization. Suitable for Vaccinia virus (VACV).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityVaccinia virus (VACV)
ApplicationsELISA, Neutralization
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDA27D7
Target Protein A33, VACWR156, A33R
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
RRID Anti-Vaccinia virus(VACV) A33R Antibody (A27D7) (abinScience Cat# VK465013, RRID:AB_3725713)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Protein OPG161 (A33R) is a ~20 kDa protein. Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.

1. Wolffe, EJ. et al. (2001) Journal of virology 75, 303-10. PMID: 11119600
2. Breiman, A. et al. (2013) The Journal of general virology 94, 720-725. PMID: 23255618
3. Monticelli, SR. et al. (2020) Journal of virology 94. PMID: 31941777
Note For research use only.
Images
  • Anti-Vaccinia virus(VACV) A33R Recombinant Antibody (A27D7)

    SDS-PAGE

    SDS-PAGE for Anti-Vaccinia virus(VACV) A33R Antibody (A27D7).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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