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Anti-TCR αβ Reference Antibody (SAA0485, RUO) (HP370013)

Anti-TCR αβ Reference Antibody (SAA0485, RUO)
Anti-TCR αβ Reference Antibody (SAA0485, RUO)
Anti-TCR αβ Reference Antibody (SAA0485, RUO)
Anti-TCR αβ Reference Antibody (SAA0485, RUO)
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  • Overview

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  • References

  • Datasheet

  • SDS

Overview
Catalog No.HP370013
Description
Anti-TCR αβ/alpha-beta TCR (SAA0485) (HP370013) is a research-grade recombinant antibody targeting TCR αβ. Produced in mammalian cells with native-like glycosylation.
Highlights
  • Research Grade — For PK/PD studies, assay development, and ADA research.
  • Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA0485
Expression system Mammalian cells
Clonality Monoclonal
Tested applications FCM
Target TCR αβ
Endotoxin level < 10 EU/mg
Purity >95% purity as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only. Not suitable for clinical or therapeutic use.
Images
  • Anti-TCR αβ Reference Antibody (SAA0485, RUO)

    Flow Cytometry

    Flow-cytometry using anti-human TCR αβ antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human TCR αβ antibody monoclonal antibody (Catalog # HP370013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-TCR αβ Reference Antibody (SAA0485, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Anti-TCR αβ/alpha-beta TCR (SAA0485)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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