Anti-Human CDCP1 Reference Antibody (RG7287, RUO) (HV451016)

Name: Anti-Human CDCP1 Reference Antibody (RG7287, RUO)
Brand: abinScience
SKU: HV451016
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HV451016
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	RG7287
Expression system	Mammalian cells
Clonality	Monoclonal
Target	CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9H5V8
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	RG7287
Background	CUB domain-containing protein 1 (CD318/CDCP1) is a ~92 kDa protein. May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
References	1. Gwendlyn Kollmorgen, et al. Antibody mediated CDCP1 degradation as mode of action for cancer targeted therapy. Mol Oncol. 2013 Dec;7(6):1142-51. [HV451016]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects CD318 in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD318/CDCP1 (RG7287).
Flow CytoMetry

Flow-cytometry using APC anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD318 monoclonal antibody (Catalog: HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD318 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD318 monoclonal antibody (Catalog HV451016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.