Anti-Human OSM/Oncostatin-M Reference Antibody (GSK315234, RUO) (HB745026)

Name: Anti-Human OSM/Oncostatin-M Reference Antibody (GSK315234, RUO)
Brand: abinScience
SKU: HB745026
Price: 176 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HB745026
Description	Anti-Human OSM/Oncostatin-M (GSK315234) (HB745026) is a research-grade recombinant antibody targeting Oncostatin-M. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	GSK315234
Expression system	Mammalian cells
Clonality	Monoclonal
Target	Oncostatin-M, OSM
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13725
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GSK315234
Background	Oncostatin-M (OSM) is a ~28 kDa protein. Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST).
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Western blot

Western blot analysis was performed using anti-OSM monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OSM (Catalog No: HB745011)
Lane 2: negative control
Bioactivity

Detects recombinant human OSM/Oncostatin-M (Catalog No: HB745011) in indirect ELISA
Flow cytometry

Flow-cytometry using anti-human OSM antibody. KG-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human OSM monoclonal antibody (Catalog HB745026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.