Research Grade Anti-Human CD172a/SIRPA (CC-95251) (HX892066)

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Spec:

100ug
1mg

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Datasheet

Overview

Catalog No.	HX892066
Description	Anti-Human CD172a/SIRPA (CC-95251) (HX892066) is a research-grade recombinant antibody targeting CD172. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	CC-95251
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	BIT, Signal-regulatory protein alpha-1, Brain Ig-like molecule with tyrosine-based activation motifs, MFR, SIRP, Sirp-alpha-1, Sirp-alpha-2, PTPNS1, SIRPA, SHP substrate 1, CD172 antigen-like family member A, Sirp-alpha-3, Inhibitory receptor SHPS-1, Signal-regulatory protein alpha-3, CD172a, Tyrosine-protein phosphatase non-receptor type substrate 1, MyD-1 antigen, Bit, SHPS-1, Macrophage fusion receptor, Signal-regulatory protein alpha-2, SHPS1, MYD1, p84
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P78324
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	CC-95251, CC95251, CC95251
Background	Tyrosine-protein phosphatase non-receptor type substrate 1 (CD172a/SIRPA) is a ~54 kDa protein. Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. 1. Yao, M. et al. (2014) Journal of the American Society of Nephrology : JASN 25, 1171-86. PMID: 24511121
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD172a/SIRPA (CC-95251).
Western blot

Western blot analysis was performed using anti-CD172a/SIRPA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD172a/SIRPA (Catalog No: HX892011)
Lane 2: negative control
Bioactivity

Detects recombinant CD172a (Catalog No: HX892011) in indirect ELISA.