Recombinant Human CD172a/SIRPA Protein, C-His (HX892011) expressed in Mammalian Cells, spanning Met1-Arg370.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
P78324
Protein length
Met1-Arg370
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Tested applications
WB
Target
BIT, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SIRP, SIRPA, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Predicted molecular weight
41.14 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
BIT, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SIRP, SIRPA, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84
Background
Tyrosine-protein phosphatase non-receptor type substrate 1 (CD172a/SIRPA) is a ~54 kDa protein. Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin.
1. Yao, M. et al. (2014) Journal of the American Society of Nephrology : JASN 25, 1171-86. PMID: 24511121
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CD172a/SIRPA Protein, C-His
Bioactivity
Detects Human SIRPA Antibody in indirect ELISAs.
Bioactivity
Detects Human CD172a/SIRPA Antibody (18D5) in indirect ELISAs.
Bioactivity
Detects Human CD172a/SIRPA Antibody (SAA1393) in indirect ELISAs.
Western blot
Detected by Anti-CD172a/SIRPA monoclonal antibody (Catalog No: HX892066)
Bioactivity
Detected by CC-95251 (Catalog No: HX892066) in indirect ELISA.
Bioactivity
Detected by Epacmarstobart (Catalog No: HX892036) in indirect ELISA.
Bioactivity
Detected by Lumistobart (Catalog No: HX892116) in indirect ELISA.
Bioactivity
Immobilized HC359021 Human CD47 Recombinant Protein (C-Fc) at 2 μg/mL(100 μL/well) can bind HX892011 Human CD172a/SIRPA Recombinant Protein (C-His), the EC 50 is 793 ng/mL.
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