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Overview
Catalog No.JN842012
DescriptionPeptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins
Expression systemE. coli
Protein lengthPNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
ApplicationsRemoval of high mannose N-glycans from glycoproteins
SpeciesElizabethkingia miricola
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Predicted molecular weight 37.08 kDa
Form Liquid
Storage buffer 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternate Names83534-39-8
Note For research use only.
Images
  • SDS-PAGE

    SDS-PAGE for PNGase F

  • Bioactivity

    Deglycosylation of Human IgG1 Fc by PNGase F.

  • Bioactivity

    Schematic diagram of PNGase F cleavage site.

Datasheet
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