Human FGF1 Recombinant Protein (HY232012)

Name: Human FGF1 Recombinant Protein
Brand: abinScience
SKU: HY232012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY232012
Description	Recombinant Human FGF1 Protein (HY232012) expressed in E. coli, spanning Met1-Asp155. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P05230
Protein length	Met1-Asp155
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	17.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Endothelial cell growth factor, HBGF-1, Fibroblast growth factor 1, FGF1, ECGF, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, FGF-1, FGFA
Background	Fibroblast growth factor 1 (FGF1/aFGF) is a ~17 kDa protein. Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. 1. Mori, S. et al. (2008) The Journal of biological chemistry 283, 18066-75. PMID: 18441324 2. Yamaji, S. et al. (2010) PloS one 5, e10273. PMID: 20422052 3. Mori, S. et al. (2013) PloS one 8, e57927. PMID: 23469107
Note	For research use only.

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SDS-PAGE for Recombinant Human FGF1 Protein

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Datasheet

Human FGF1 Recombinant Protein.pdf

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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

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Human FGF1 Recombinant Protein (HY232012)

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