Human CFI Recombinant Protein (N-His) (HY223012)

Name: Human CFI Recombinant Protein (N-His)
Brand: abinScience
SKU: HY223012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY223012
Description	Recombinant Human CFI Protein, N-His (HY223012) expressed in E. coli, spanning Lys19-Ile335. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P05156
Protein length	Lys19-Ile335
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Complement factor I, CFI, C3B/C4B inactivator, IF
Background	Complement factor I (CFI) is a ~65 kDa protein. Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization. 1. Timár, KK. et al. (2007) Molecular immunology 44, 2943-9. PMID: 17320177 2. Harrison, RA. et al. (1980) Molecular immunology 17, 9-20. PMID: 7360115 3. Barilla-LaBarca, ML. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 6298-304. PMID: 12055245 4. Seya, T. et al. (1990) Journal of biochemistry 107, 310-5. PMID: 2141838 5. Sahu, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 5596-604. PMID: 9605165 6. Xue, X. et al. (2017) Nature structural & molecular biology 24, 643-651. PMID: 28671664
Note	For research use only.

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SDS-PAGE for Recombinant Human CFI Protein, N-His

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Datasheet

Human CFI Recombinant Protein (N-His).pdf

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Human CFI Recombinant Protein (N-His) (HY223012)

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