Human UNG Recombinant Protein (N-His) (HY404012)

Name: Human UNG Recombinant Protein (N-His)
Brand: abinScience
SKU: HY404012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY404012
Description	Recombinant Human UNG Protein, N-His (HY404012) expressed in E. coli, spanning Ala45-Leu313. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P13051
Protein length	Ala45-Leu313
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.43 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DGU, Uracil-DNA glycosylase, UNG15, UDG, UNG, UNG1
Background	Uracil-DNA glycosylase (UNG) is a ~34 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication. 1. Imai, K. et al. (2003) Nature immunology 4, 1023-8. PMID: 12958596 2. Kavli, B. et al. (2005) The Journal of experimental medicine 201, 2011-21. PMID: 15967827 3. Akbari, M. et al. (2007) Neuroscience 145, 1201-12. PMID: 17101234 4. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144 5. Mol, CD. et al. (1995) Cell 82, 701-8. PMID: 7671300 6. Slupphaug, G. et al. (1996) Nature 384, 87-92. PMID: 8900285 7. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624 8. Bharati, S. et al. (1998) Nucleic acids research 26, 4953-9. PMID: 9776759
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human UNG Protein, N-His

References

HY404012-SDS-PAGE-1.jpg

Datasheet

Human UNG Recombinant Protein (N-His).pdf

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Human UNG Recombinant Protein (N-His) (HY404012)

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