Human CFL1 Recombinant Protein (N-His) (HB213012)

Name: Human CFL1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB213012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB213012
Description	Recombinant Human CFL1 Protein, N-His (HB213012) expressed in E. coli, spanning Ser3-Leu161. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P23528
Protein length	Ser3-Leu161
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.07 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cofilin, non-muscle isoform, CFL1, p18, 18 kDa phosphoprotein, CFL, Cofilin-1
Background	Cofilin-1 (CFL1) is a ~18 kDa protein. Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. 1. Yeoh, S. et al. (2002) Journal of molecular biology 315, 911-25. PMID: 11812157 2. Gohla, A. et al. (2005) Nature cell biology 7, 21-9. PMID: 15580268 3. Bai, SW. et al. (2011) BMC biology 9, 54. PMID: 21834987 4. Borroni, EM. et al. (2013) Science signaling 6, ra30.1-11, S1-3. PMID: 23633677
Note	For research use only.

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SDS-PAGE for Recombinant Human CFL1 Protein, N-His

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Datasheet

Human CFL1 Recombinant Protein (N-His).pdf

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Human CFL1 Recombinant Protein (N-His) (HB213012)

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