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Recombinant Human CFP Protein, N-His (HB792012)

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Recombinant Human CFP Protein, N-His

Recombinant Human CFP Protein, N-His

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Datasheet

Overview

Catalog No.	HB792012
Description	Recombinant Human CFP Protein, N-His (HB792012) expressed in E. coli, spanning Gln65-Cys190. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P27918
Protein length	Gln65-Cys190
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.26 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PFC, CFP, Properdin, Complement factor P
Background	Properdin (CFP) is a ~51 kDa protein. A positive regulator of the alternate pathway (AP) of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b). 1. Hourcade, DE. (2006) The Journal of biological chemistry 281, 2128-32. PMID: 16301317 2. Ferreira, VP. et al. (2010) Immunobiology 215, 932-40. PMID: 20382442 3. Pedersen, DV. et al. (2017) The EMBO journal 36, 1084-1099. PMID: 28264884 4. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277 5. Pedersen, DV. et al. (2019) Frontiers in immunology 10, 2007. PMID: 31507604
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CFP Protein, N-His

References

HB792012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human CFP Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

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Working Solution

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Recombinant Human CFP Protein, N-His