Human GABRA2 Recombinant Protein (N-GST) (HW333012)

Name: Human GABRA2 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HW333012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW333012
Description	Recombinant Human GABRA2 Protein, N-GST (HW333012) expressed in E. coli, spanning Phe42-Ile250. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P47869
Protein length	Phe42-Ile250
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	50.87 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GABA(A) receptor subunit alpha-2, GABRA2, Gamma-aminobutyric acid receptor subunit alpha-2
Background	Gamma-aminobutyric acid receptor subunit alpha-2 (GABRA2) is a ~51 kDa protein. Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interfaces. When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. The alpha-2 subunit exhibits synaptogenic activity together with beta-2 and very little to no activity together with beta-3, the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. 1. Bonnert, TP. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 9891-6. PMID: 10449790 2. Butler, KM. et al. (2018) Brain : a journal of neurology 141, 2392-2405. PMID: 29961870 3. Maljevic, S. et al. (2019) Brain : a journal of neurology 142, e15. PMID: 31032849
Note	For research use only.

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SDS-PAGE for Recombinant Human GABRA2 Protein, N-GST

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Datasheet

Human GABRA2 Recombinant Protein (N-GST).pdf

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Human GABRA2 Recombinant Protein (N-GST) (HW333012)

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