Human CDC42 Recombinant Protein (N-His) (HX069012)

Name: Human CDC42 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX069012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX069012
Description	Recombinant Human CDC42 Protein, N-His (HX069012) expressed in E. coli, spanning Met1-Cys188. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P60953
Protein length	Met1-Cys188
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.1 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CDC42, G25K GTP-binding protein, Cell division control protein 42 homolog
Background	Cell division control protein 42 homolog (CDC42) is a ~21 kDa protein. Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. 1. Oceguera-Yanez, F. et al. (2005) The Journal of cell biology 168, 221-32. PMID: 15642749 2. Modzelewska, K. et al. (2006) The Journal of biological chemistry 281, 37527-35. PMID: 17038317 3. Hong, IK. et al. (2012) The Journal of biological chemistry 287, 32027-39. PMID: 22843693 4. Gauthier-Campbell, C. et al. (2004) Molecular biology of the cell 15, 2205-17. PMID: 14978216 5. Majmundar, AJ. et al. (2021) Science advances 7. PMID: 33523862 6. Schlam, D. et al. (2015) Nature communications 6, 8623. PMID: 26465210 7. Ninomiya, K. et al. (2021) Journal of cell science 134. PMID: 33310911
Note	For research use only.

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SDS-PAGE for Recombinant Human CDC42 Protein, N-His

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Datasheet

Human CDC42 Recombinant Protein (N-His).pdf

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Human CDC42 Recombinant Protein (N-His) (HX069012)

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