ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
69.51 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Histone-lysine N-methyltransferase EZH2 is a ~85 kDa protein. Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems.
1. Bracken, AP. et al. (2003) The EMBO journal 22, 5323-35. PMID: 14532106
2. Cao, R. et al. (2004) Molecular cell 15, 57-67. PMID: 15225548
3. Pasini, D. et al. (2004) The EMBO journal 23, 4061-71. PMID: 15385962
4. Bracken, AP. et al. (2006) Genes & development 20, 1123-36. PMID: 16618801
5. Kim, DH. et al. (2006) Nature structural & molecular biology 13, 793-7. PMID: 16936726
6. Bracken, AP. et al. (2007) Genes & development 21, 525-30. PMID: 17344414
7. McCabe, MT. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2989-94. PMID: 22323599
8. Chu, CS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 1355-60. PMID: 24474760
9. Kim, JM. et al. (2015) Scientific reports 5, 16714. PMID: 26581166
10. Li, D. et al. (2018) Nature communications 9, 2829. PMID: 30026490
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human EZH2 Protein, N-GST
中文
English
한국어
日本語
Español
Français
Русский
