Human RPP25 Recombinant Protein (N-His) (HP449012)

Name: Human RPP25 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP449012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP449012
Description	Recombinant Human RPP25 Protein, N-His (HP449012) expressed in E. coli, spanning Met1-Ala199. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BUL9
Protein length	Met1-Ala199
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ribonuclease P protein subunit p25, RPP25, RNase P protein subunit p25
Background	Ribonuclease P protein subunit p25 (RPP25) is a ~20 kDa protein. Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences. _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Guerrier-Takada, C. et al. (2002) RNA (New York, N.Y.) 8, 290-5. PMID: 12003489 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Welting, TJ. et al. (2006) RNA (New York, N.Y.) 12, 1373-82. PMID: 16723659 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Wu, J. et al. (2018) Cell 175, 1393-1404.e11. PMID: 30454648 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Goldfarb, KC. et al. (2017) Genes & development 31, 59-71. PMID: 28115465 _x005F_x005F_x000D_
Note	For research use only.

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SDS-PAGE for Recombinant Human RPP25 Protein, N-His

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Datasheet

Human RPP25 Recombinant Protein (N-His).pdf

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Human RPP25 Recombinant Protein (N-His) (HP449012)

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