Human TRIM27 Recombinant Protein (N-His) (HB027012)

Name: Human TRIM27 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB027012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB027012
Description	Recombinant Human TRIM27 Protein, N-His (HB027012) expressed in E. coli, spanning Ser315-Pro513. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P14373
Protein length	Ser315-Pro513
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	RING finger protein 76, Zinc finger protein RFP, RFP, TRIM27, RNF76, RING-type E3 ubiquitin transferase TRIM27, Tripartite motif-containing protein 27, Ret finger protein
Background	Zinc finger protein RFP (TRIM27) is a ~58 kDa protein. E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or autophagy. Ubiquitinates PIK3C2B and inhibits its activity by mediating the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. 1. Zurek, B. et al. (2012) PloS one 7, e41255. PMID: 22829933 2. Zaman, MM. et al. (2013) Molecular and cellular biology 33, 4971-84. PMID: 24144979 3. Cai, J. et al. (2018) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 32, 5238-5249. PMID: 29688809 4. Garcia-Garcia, J. et al. (2023) The FEBS journal 290, 1096-1116. PMID: 36111389 5. Hao, YH. et al. (2013) Cell 152, 1051-64. PMID: 23452853 6. Zheng, Q. et al. (2015) Cell research 25, 1121-36. PMID: 26358190
Note	For research use only.

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SDS-PAGE for Recombinant Human TRIM27 Protein, N-His

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HB027012-SDS-PAGE-1.jpg

Datasheet

Human TRIM27 Recombinant Protein (N-His).pdf

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Human TRIM27 Recombinant Protein (N-His) (HB027012)

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