Human ALDH3A2 Recombinant Protein (N-His) (HW301012)

Name: Human ALDH3A2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW301012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW301012
Description	Recombinant Human ALDH3A2 Protein, N-His (HW301012) expressed in E. coli, spanning Met1-Lys428. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P51648
Protein length	Met1-Lys428
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	50.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ALDH10, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, FALDH, Microsomal aldehyde dehydrogenase, Fatty aldehyde dehydrogenase, ALDH3A2
Background	Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) is a ~54 kDa protein. Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. 1. Lloyd, MD. et al. (2007) Journal of enzyme inhibition and medicinal chemistry 22, 584-90. PMID: 18035827 2. Sanders, RJ. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 2064-71. PMID: 18182499 3. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490 4. Keller, MA. et al. (2014) Nature communications 5, 4439. PMID: 25047030 5. Kelson, TL. et al. (1997) Biochimica et biophysica acta 1335, 99-110. PMID: 9133646 6. Verhoeven, NM. et al. (1998) FEBS letters 429, 225-8. PMID: 9662422
Note	For research use only.

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SDS-PAGE for Recombinant Human ALDH3A2 Protein, N-His

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Datasheet

Human ALDH3A2 Recombinant Protein (N-His).pdf

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Formula

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Human ALDH3A2 Recombinant Protein (N-His) (HW301012)

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