Human HELLS Recombinant Protein (N-His) (HV401012)

Name: Human HELLS Recombinant Protein (N-His)
Brand: abinScience
SKU: HV401012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV401012
Description	Recombinant Human HELLS Protein, N-His (HV401012) expressed in E. coli, spanning Asp523-His748. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9NRZ9
Protein length	Asp523-His748
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PASG, Lymphoid-specific helicase, SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6, SMARCA6, HELLS, Proliferation-associated SNF2-like protein
Background	Lymphoid-specific helicase (HELLS) is a ~97 kDa protein. ATP-dependent chromatin remodeler that regulates chromatin accessibility, DNA methylation, and histone modifications. It facilitates de novo DNA methylation at repetitive sequences and promotes transcriptional silencing via recruitment of DNA methyltransferases (DNMTs) and histone deacetylases (HDACs), contributing to heterochromatin formation and repression of transposable elements. Also involved in DNA repair by recruiting DNA damage response mediators to double-strand breaks in heterochromatin, promoting homologous recombination via RBBP8/CtIP-dependent end resection. During meiosis, it is recruited by PRDM9 to recombination hotspots, aiding chromatin opening. Through these diverse roles, is crucial for processes such as development, differentiation, and genomic stability. 1. Unoki, M. et al. (2019) The Journal of clinical investigation 129, 78-92. PMID: 30307408 2. Burrage, J. et al. (2012) Journal of cell science 125, 5524-34. PMID: 22946062 3. Kollárovič, G. et al. (2020) Nucleic acids research 48, 1872-1885. PMID: 31802118 4. Spruce, C. et al. (2020) Genes & development 34, 398-412. PMID: 32001511
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human HELLS Protein, N-His

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Datasheet

Human HELLS Recombinant Protein (N-His).pdf

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Human HELLS Recombinant Protein (N-His) (HV401012)

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