Human WDR24 Recombinant Protein (N-His-SUMO) (HP377012)

Name: Human WDR24 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HP377012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP377012
Description	Recombinant Human WDR24 Protein, N-His-SUMO (HP377012) expressed in E. coli, spanning Glu57-Thr158. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96S15
Protein length	Glu57-Thr158
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.93 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	WDR24, C16orf21, GATOR complex protein WDR24, WD repeat-containing protein 24
Background	GATOR2 complex protein WDR24 is a ~88 kDa protein. Catalytic component of the GATOR2 complex, a multiprotein complex that acts as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex. 1. Bar-Peled, L. et al. (2013) Science (New York, N.Y.) 340, 1100-6. PMID: 23723238 2. Wolfson, RL. et al. (2016) Science (New York, N.Y.) 351, 43-8. PMID: 26449471 3. Saxton, RA. et al. (2016) Science (New York, N.Y.) 351, 53-8. PMID: 26586190 4. Saxton, RA. et al. (2016) Nature 536, 229-33. PMID: 27487210 5. Valenstein, ML. et al. (2022) Nature 607, 610-616. PMID: 35831510 6. Jiang, C. et al. (2023) Molecular cell 83, 74-89.e9. PMID: 36528027 7. Dai, X. et al. (2023) Nature metabolism 5, 265-276. PMID: 36732624
Note	For research use only.

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SDS-PAGE for Recombinant Human WDR24 Protein, N-His-SUMO

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Datasheet

Human WDR24 Recombinant Protein (N-His-SUMO).pdf

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Human WDR24 Recombinant Protein (N-His-SUMO) (HP377012)

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