HCV NS1/gp68/gp70/Envelope glycoprotein E2 Recombinant Protein (C-His) (VK681011)

Name: HCV NS1/gp68/gp70/Envelope glycoprotein E2 Recombinant Protein (C-His)
Brand: abinScience
SKU: VK681011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	VK681011
Description	Recombinant HCV NS1/gp68/gp70/Envelope glycoprotein E2 Protein, C-His (VK681011) expressed in Mammalian Cells, spanning Glu384-Glu661. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	F5BWY6
Protein length	Glu384-Glu661
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Recombinant Hepatitis C virus H77(5'UTR-NS2)/JFH1_V787A, Q1247L
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Genome polyprotein
Background	Genome polyprotein (NS1/gp68) is a ~328 kDa protein. Cysteine protease required for the proteolytic auto-cleavage between the non-structural proteins NS2 and NS3. The N-terminus of NS3 is required for the function of NS2 protease (active region NS2-3). Promotes the initiation of viral particle assembly by mediating the interaction between structural and non-structural proteins.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant HCV NS1/gp68/gp70/Envelope glycoprotein E2 Protein, C-His
Bioactivity

Detects HCV NS1/gp68/gp70/Envelope glycoprotein E2 Antibody (HC33.1) in indirect ELISAs.
Western blot

Detected by Anti-HCV NS1 monoclonal antibody (Catalog No: VK681020)
Western blot

Detected by Anti-HCV NS1 monoclonal antibody (Catalog No: VK681016)
Western blot

Detected by Anti-HCV NS1 monoclonal antibody (Catalog No: VK681283)
Bioactivity

Detected by Research Grade Anti-HCV NS1/Envelope glycoprotein E2 (MBL-HCV1) (Catalog No: VK681016) in indirect ELISA
Bioactivity

Detected by InVivoMAb Anti-HCV NS1/gp68/gp70/E2 Antibody (HC84.26) (Catalog No: VK681020) in indirect ELISA
Bioactivity

Detected by AP33 (Catalog No: VK681283) in indirect ELISA.

References

VK681011-SDS-PAGE-1.jpg

Datasheet

HCV NS1/gp68/gp70/Envelope glycoprotein E2 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

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Recombinant Proteins

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HCV NS1/gp68/gp70/Envelope glycoprotein E2 Recombinant Protein (C-His) (VK681011)

Overview

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References

Datasheet

SDS

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